Engineering CYP153A M.aq to Oxyfunctionalize its Inhibitor Dodecylamine Using a LC/MS Based Rapid Flow Analysis Screening
نویسندگان
چکیده
The catalytic space of the P450 monooxygenase CYP153AM.aq was opened from a terminal (ω-) fatty acid hydroxylase to catalyst capable performing ω-hydroxylation dodecylamine, which is potent inhibitor for wild-type enzyme. A simple screening method named Rapid-flow Analysis Product Peaks (RAPP) established and applied measure saturation libraries directly 96-deepwell plate in 36 seconds per sample. obtained variants are less inhibited by amine, although concurrently show affinity towards acid. Molecular modelling molecular dynamics simulations showed significant effects mutations on substrate tunnel architectures.
منابع مشابه
In vitro flow cytometry-based screening platform for cellulase engineering
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 10(7) events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during the transformation of mutant libraries ...
متن کاملMAQ – A Bioinformatics Tool for Automatic Macroarray Analysis
This article presents a prototype of an application for the analysis of cDNA/gene expression data and contribution for genome annotation from macroarrays. The application described here allows the quantification of a set of experiments with macroarrays of the same size. It generates html reports, easily shared, showing the expression of genes contained in each membrane and a global report compa...
متن کاملLCMS-96_Identifying psychotropics using Toxtyper _2014.indd
Various instrument types and LC-MS approaches have been explored in an attempt to develop a comprehensive analysis method for detecting and definitely identifying as many compounds as possible in a single run. However, everyday analyses often require the detection of a defined set of substances (for example, hypnotics in cases of drugfacilitated assault (DFA). The aim of this project was to dev...
متن کاملapplying transitivity theory to gender analysis of efl textbook: : a comparative study.
efl/esl textbooks have been regarded as essential language teaching materials with which the learners spend about 70 up to 90 percent of their class time. the important role they play and their vast use make them not only influential in learning the language but also in shaping values and attitudes. put it another way, textbooks socialize learners using their contents (i.e. texts, illustrations...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemcatchem
سال: 2022
ISSN: ['1867-3899', '1867-3880']
DOI: https://doi.org/10.1002/cctc.202101648